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The present study was undertaken to observe the viability of excysted metacercariae of Clonorchis sinensis in vitro when the larvae were incubated in the bile (Bbile) of cattle, dog, pig and fowl at 37℃. The survival rate and 50% lethal time of the incubated larvae were investigated. The results obtained were as follows: 1. The survival rates decreased according to the chronology of incubation in all kinds of bile juice. At first, rapid decline of the survival rates in various animal bile appeared during 412 hours after the incubation; i.e., 9 hours in cattle bile, 6 hours in the dog, 4 hours in the pig, and 12 hours in the fowl. On the other hand, the survival rate of control in saline decreased gradually during the incubation. 2. The maximum survival times of excysted metacercariae in various animal bile were 18 hours in cattle, 15 hours in dog and fowl, 12 hours in pig, whereas the larvae survived 48 hours in saline solution. 3. The 50% lethal times calculated by the formula of ReedMuench(1938) were as follows; 11 hours 18 minutes in cattle bile; 11 h. 54 min. in fowl; 7 h. 51 min. in dog; 7 h. 48 min. in pig; and 20 h. 18 min. in the saline.
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